| abstract |
In one embodiment, methods of producing a population of differentiated target cells from a population of undifferentiated pluripotent stem cells (PSC) are provided herein. Such methods may include culturing the population of undifferentiated PSCs, such as human embryonic stem cells (hESC), on an attachment matrix which comprises at least two or more laminin isoforms. The two or more laminin isoforms may include a laminin combination of one or more laminin isoforms that support the hESC cells; and one or more laminin isoforms that would support a population of differentiated target cells. The one or more laminins (LN) that support the hESC cells may be selected from LN-511 or LN-521, while the population of differentiated target cells is a population of cardiomyocytes, and the one or more laminins that would support the cardiomyocytes are selected from LN-411, LN-111, LN-421, LN-211, LN-332, or LN-121. |