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http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
filingDate 2013-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1a010862ce835c3212c9e1db46f20bf
publicationDate 2013-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2013288380-A1
titleOfInvention Method for detecting formation of g-quadruplex
abstract It is an object of the method of the present invention to specifically detect a G-quadruplex even in the presence of potassium ions. n The method of the present invention determines whether a target DNA forms a G-quadruplex using a phenomenon in which thioflavin T generates a strong fluorescence when reacted with the G-quadruplex in the presence of potassium ions. In the method of the present invention, a first sample solution containing potassium ions and a target DNA is retained under such conditions that a G-quadruplex is formed, followed by adding thioflavin T and measuring a first fluorescence intensity value. A second sample solution containing the target DNA is retained under conditions for the G-quadruplex to be destabilized, thioflavin T is added, a second fluorescence intensity value is measured, and it is determined that the target DNA can form a G-quadruplex if a difference between the first fluorescence intensity value and the second fluorescence intensity value is 0 or more.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112080549-A
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11033571-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020111753-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019240503-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019240504-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11175285-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105717081-A
priorityDate 2011-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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