Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_57f3e2af72b2933c004ed61dfccaaee1 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-583 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate |
2013-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_458b788163675b839ba806a8d756ed91 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fc9ee94e58629db06821b805ea6a78d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11bcadd825f55f65ce0320004c87615d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56d0fbac9f5486c46204d871ef90e3e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acca4c8395019cc7ba4e07a22e66bf54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df4089308afc4224ba367dcdf02e8581 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e9f2b22b3ea86919831296b97ad4b2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e51f0251f7b2e31c9b3da575dc16789 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d67b4ca4ffff98b55f949cc22e8907f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc744e9b3eaa40f5d6da85348d7dbd69 |
publicationDate |
2013-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2013273540-A1 |
titleOfInvention |
Method for chromogenic detection of two or more target molecules in a single sample |
abstract |
The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021226311-A1 |
priorityDate |
2008-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |