| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ead6003549e8523ff3b6947461abc4b0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8133e6e1a34a40a7b1b1e59e695c372
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41651508566f0385833e1ba14a21ffd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b6fade35157316c87c730e5639f7965c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2012-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7a69718c153f78219079608750f2199
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ddf1c1bf4681e9a7ec936418fdb92c2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce9c1314ba3841e21eff45b15ec37514 |
| publicationDate |
2013-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2013273526-A1 |
| titleOfInvention |
DNA Polymerases Having Improved Labeled Nucleotide Incorporation Properties |
| abstract |
The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions are located at portions of the O-helix, the K-helix, and the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. n The invention also provides polynucleotides encoding the mutant DNA polymerases, optionally including expression vectors for mutant polymerase recombinant production, and further optionally including host cells containing the polynucleotides. Numerous methods of using the subject DNA polymerases are provided, including uses for chain termination sequencing and PCR. Kits are provided for synthesizing fluorescently labeled polynucleotides and may include a mutant DNA polymerase of the invention and a fluorescently labeled nucleotide. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11198906-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10626456-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9296999-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10358634-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10815465-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE47476-E
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9873911-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-RE47177-E
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9476035-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11377644-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9758774-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9399766-B2 |
| priorityDate |
1997-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |