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filingDate 2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2013-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2013260373-A1
titleOfInvention Quantification of Human Mitochondrial DNA Using Synthesized DNA Standards
abstract A real-time quantitative PCR assay that utilizes a duplex, synthetic DNA standard to ensure optimal quality assurance and quality control. One embodiment of the invention facilitates amplification of mtDNA by focusing on a 105-base pair target sequence that is minimally homologous to non-human mtDNA. The present invention can also be used to identify the presence of PCR inhibitors and thus indicate the need for sample repurification.
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