| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4120caaf4997cf81d06cd4d834d404eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c29324d6e4cbd44de52b26a0bb7e5ce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46d6659ac4ce14165ae78eb9c154d6ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b24e2d499e198f886baf2d81b739ca08 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-111
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3519
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-12 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate |
2011-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4d568378e8eabb4be81d6a3f22e75e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_222795e0482ae85dcb7adce00de9bd27
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eabf71a73a2814855bb245b19192b4c9 |
| publicationDate |
2013-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2013244239-A1 |
| titleOfInvention |
Nucleic acid construct and complex formation method and screening method using the same |
| abstract |
The present invention is to provide a new detection method that is superior in detection sensitivity and allows a simple operation. A nucleic acid construct that includes a cloning region, an encoding nucleic acid of a peptide tag, an encoding nucleic acid of an aptamer that is bindable to the peptide tag is used. By inserting the encoding nucleic acid of arbitrary peptide into the cloning region of the nucleic acid construct, the nucleic acid construct is expressed in vivo. Thereby, a complex of a fusion transcript having the base sequence that includes the encoding nucleic acid of arbitrary peptide, the encoding nucleic acid of a peptide tag, and the encoding nucleic acid of the aptamer and a fusion translation that includes the arbitrary peptide and the peptide tag is formed. By bringing the complex into contact with a target and recovering the complex that is bound to the target, the peptide that is bindable to a target and its encoding nucleic acid can be identified from the transcript of the encoding nucleic acid of arbitrary peptide in the complex. |
| priorityDate |
2010-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |