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publicationDate 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2013167270-A1
titleOfInvention Method for differentiating fertile and sterile plant lines by detection of polymorphic markers in chloroplast dna
abstract This technology is generally related to a method for differentiating fertile and sterile plant lines at the DNA level, by detecting polymorphisms in chloroplast DNA and compositions and uses thereof. More specifically, the technology is directed to a method for detecting plant line contamination by detecting polymorphic markers targeting simple sequence repeats (SSRs) that distinguish normal fertile plant lines from cytoplasmic male sterile (cms) plant lines in chloroplast DNA. This method may be applied to plants that have not yet flowered or to plant seeds. The technology also relates to a method of mitigating seed contamination based on this differentiation method by selecting plants essentially free of contamination or rejecting a plant if it exhibits contamination. The technology also relates to a plant seed lot produced by these same processes.
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