http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013130940-A1

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filingDate 2011-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d4f6f4efc48d3b10da36209c853ea98
publicationDate 2013-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2013130940-A1
titleOfInvention Multi-targeted priming for genome-wide gene expression assays
abstract The present invention is a method of generating a library of expressed cDNA sequences using multi-targeted primers that are complementary to degenerate motifs present in a large proportion of corresponding mRNA sequences. Multi-targeting priming (MTP) for genome-wide gene expression assays provides selective targeting of multiple sequences and counter-selection against undesirable sequences. Priming with MTPs in addition to oligo-dT can result in higher sensitivity, a greater number of genes whose expression is well measured, a greater number of genes whose differences in gene expression are detected to be statistically, and a greater power to detect meager differences in expression.
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