http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013095496-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b43cd7382a8b607a90da7f83e264e5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efd09c296645384c4e1c770411f7aa01 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2011-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_038b3cc86d2510caadf9cd299278fd23 |
| publicationDate | 2013-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2013095496-A1 |
| titleOfInvention | Soluble Quencher to reduce Background in qPCR assays |
| abstract | Soluble Quencher to reduce Background in qPCR assays The invention is in the field of is in the field of analytical technology. In particular, it is useful for conducting (RT-)quantitative PCR (qPCR) reactions for detection of DNA and RNA involving fluorescent probes. The distinguishing feature of the invention is to decrease the background not of an individual probe, but of all probes carrying the same fluorescent label. This is achieved by adding a soluble quenching dye to the qPCR reaction mix. The soluble quenching dye has an absorption of at least 40% of its maximal absorbance at the excitation wavelength and/or at the emission wave length of the fluorescent dye label This dye then acts as a “soluble shield” and allows to reduce the fluorescent background brought in by a large number of identically labeled probes. Depending on the nature (i.e. absorption spectrum) of the quenching dye, it is possible to selectively reduce the background of a single detection channel, while maintaining the signal strength of the other detection channels: This provides more flexibility, in case not all channels exhibit the same high background. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018334715-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11773443-B2 |
| priorityDate | 2010-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.