http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013071848-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_724ad9c6de586cef9a0b7592d64430e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81306fe8e39a7b2308b445d9d4573d5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36f6307d322cd2f9680567907c0d106e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-114 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ef99d00403d92fc0ce4a97dc79f22c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17830b8fcea379f61dae36e34ae6d32c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17923202345f74657d995cc2912db049 |
| publicationDate | 2013-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2013071848-A1 |
| titleOfInvention | One-step method for quantitative determination of uracil in DNA by real-time PCR |
| abstract | Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The archaeal DNA polymerase from Pyrococcus furiosus binds strongly to the deaminated base uracil and stalls on uracil-containing templates. Here, we present a straightforward method for quantitative assessment of uracil in DNA within specific genomic segments. We use wild type Pyrococcus furiosus polymerase in parallel with its point mutant version which lacks the uracil-binding specificity on synthetic and genomial DNA samples to quantify the uracil content in a single-step real-time PCR assay. Quantification of the PCR results is based on an approach analogous to template copy number determination in comparing different samples. Data obtained on synthetic uracil-containing templates are verified by direct isotopic measurements. |
| priorityDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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