| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_864a4b4760755a3a4301d6a8cd50e3b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8fc0668fd5cbfe23cea7d9bdd5de932a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff2309fca3a1cc3e6b6b38a2806365f2 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B20-00 |
| filingDate |
2010-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a3ae5d8afcc3b82f8cada77ce18a777
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64484b4b4cb85ffea10f5abdbd9c6b5b |
| publicationDate |
2013-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2013059734-A1 |
| titleOfInvention |
Epigenetic analysis |
| abstract |
Provided herein are methods for the analysis of methylation in nucleic acid molecules, comprising bisulfite conversion and subsequent copying or amplification in the presence of a ligand-labelled dCTP or a ligand-labelled dGTP incorporated at the site of a cytosine or complementary guanine, respectively, at positions corresponding to methylated cytosines in the bisulfite-treated nucleic acid or after the copying or amplification, subjecting the resulting nucleic acid molecules to restriction endonuclease digestion with an enzyme which comprises a cytosine nucleotide in its target recognition sequence to generate nucleic acid fragments with termini proximal or adjacent to one or more cytosines and attaching a ligand or oligonucleotide adaptor and subsequently amplifying the nucleic acid molecules. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11254985-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11410750-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725230-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11371090-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725251-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110923301-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11685958-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11174511-B2 |
| priorityDate |
2009-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |