| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_333760f168736b781d09c33fc0882b8f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f7a02d2bcccdbe190ed1d7fe3ca88cb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9776ef21e0baaba775b8c5b4ebc71f39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3a9be327d43bec557d3f1c695b372bca |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
| filingDate |
2011-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac2484ebfab1abd5c7b3877cb4c8b9a4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2854a44c7df7c0eb7b03625a6b432363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2346069caf1df7f3e0d8445dae6428e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f30c93e3133b130b8275bda88f1ba61 |
| publicationDate |
2012-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012322093-A1 |
| titleOfInvention |
Method for the determination of sequence variants of polypeptides |
| abstract |
The current invention is directed to a method for determining amino acid sequence mutations in a produced polypeptide, comprising the following steps of a) providing a sample of a produced polypeptide, b) incubating the polypeptide in the sample with a protease, c) performing a two dimensional analysis using reversed phase chromatography coupled with a high resolution mass spectroscopy (FT-ICR/FT-orbitrap) and MS/MS analysis of the amino acid sequence fragments of the peptides, d) data evaluation by comparing the LC-MS data sets obtained for the samples side by side with the data set of a reference sample, by searching for differences in the signal intensities at given retention times and by evaluation of differential signals with respect to amino acid sequence mutations. The reference sample for data evaluation (d) can be either a well characterized standard or one of the samples to be analyzed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10781484-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019051103-A1 |
| priorityDate |
2010-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |