| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_addf9108e0141eb652310061b340b9da
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_829870c3d1719dc25f6939097d79a0e3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c588997ee7d46fd58c65eef5ed0ed61a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff12915e4d1ce5442d479bd5a88d2e70 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate |
2012-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0cf9b7a6ee96736242cdfd374fab5b99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e91ba7f28413d0dd6140c6a5c374c1c7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28917c5eb244abefb0443f40da5002a8 |
| publicationDate |
2012-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012282699-A1 |
| titleOfInvention |
Mutagenesis method using polyethylene glycol mediated introduction of mutagenic nucleobases into plant protoplasts |
| abstract |
Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11434494-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019204226-A1 |
| priorityDate |
2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |