| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_667f3f5028a3a6f82f3ee5641a5b4079
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8cde1ce093b94b621f2faf21b7a4c4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d10a3febbe32d53936907e555348aea9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a4f749dd4a6bdfd2b6f858bfae76b562 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-206
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4732
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate |
2011-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3fbf09c093d3b9539fc5dc7c111f195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbd94da084d107ef8c1a9492eb04193b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8df179075a551775149a88be7bfefc0 |
| publicationDate |
2012-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012264814-A1 |
| titleOfInvention |
Isolation, Cloning, Sequencing and Functional analysis of ss-casein promoter along with the regions of exon1, intron1 and exon2 using mammary gland derived cell line of Buffalo (Bubulus bubalis) |
| abstract |
The present invention relates to a method of in vitro isolation of buffalo β-caesin promoter (buCSN2) along with the regions of exon1, intron1 and exon2 from the genomic DNA in vitro ( Bubalus bubalis ) and its functional activity in using mammary cell line. The novel buffalo β-caesin promoter along with exon1, intron1 and exon2 is isolated and cloned upstream of the Enhanced Green flourescence protein (EGFP) gene and sequenced. The transfection of the DNA construct resulted into production of EGFP protein in mammary cell lines, confirming bioactivity of this newly isolated buffalo promoter sequence. More specifically, the present invention relates to isolation, cloning, sequencing and functional analysis of the buffalo β-casein promoter in vitro using mammary cell line. |
| priorityDate |
2011-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |