http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012252049-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c589ed0ad4a2876cb5341126fe88153 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2cae285a134e674067a884b4baf1dde5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f87624dc61e308fc66ed476f31b2653 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-643 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 |
filingDate | 2010-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00d5129557f1f8384f49e2071ff97a1a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae413dfa69224a51b713373307eae8db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_261beb8b6021956a86b4bc1ca2f98a3e |
publicationDate | 2012-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2012252049-A1 |
titleOfInvention | Methods of detecting microorganisms and kits therefore |
abstract | A method of detecting microorganisms in a test sample is provided. The method includes the steps of: a) incubating the test sample with a growth media to form an incubated sample, wherein the growth media includes an enzyme substrate and the enzyme substrate includes an enzymatically hydrolysable group and a fluorescent group, wherein microorganisms present in the test sample include an enzyme that hydrolyzes the hydrolysable group from the fluorescent group to form a fluorescently detectable product, wherein the fluorescently detectable product has both an acidic and basic species; b) exciting the fluorescently detectable product with light having a wavelength of Exλiso for a time sufficient for the fluorescently detectable product to emit light, wherein Exλiso is the absorbance isosbestic point of the fluorescently detectable product; and c) detecting light emitted at a wavelength of Emλ1. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103343158-A |
priorityDate | 2009-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 504.