http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012238726-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bf0d08e88fd66249f5be2e1678549fa2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e7e0e42cba4bee6c48312821708b5d3a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-706 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-28 |
filingDate | 2012-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d3ac2a247721ce8f8f6da35a32833f0 |
publicationDate | 2012-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2012238726-A1 |
titleOfInvention | Mutant blue fluorescent protein and method of using the same for fluorescence resonance energy transfer and blue fluorescent fish |
abstract | The present invention discloses a mutant blue fluorescent protein (BFP), mutated by an error-prone PCR method or a DNA shuffling method with using a BFPvv D7 of SEQ ID NO:2 as parents, obtained from a wild type blue fluorescent protein BfgV of SEQ ID NO:1, obtained from Vibrio vulnificus , wherein a set of mutation positions of the mutant BFP corresponding to SEQ ID NO:2 comprises position 176 and position 178. In a preferred embodiment, the set of mutation positions of the mutant BFP corresponding to SEQ ID NO:2 comprises a S176R mutation or a V178I mutation. Moreover, methods of using the blue fluorescent proteins from Vibrio vulnificus for fluorescence resonance energy transfer (FRET) and a blue fluorescent fish are also provided. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102012755-B1 |
priorityDate | 2009-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 157.