| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb2c3c9b06e06ec72f0950f7961a734e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_166761b1c94ee0a8a3b9d4a1b36c2cf3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b25dac2b38b5665fbd2fbc8f4da19297
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_92ee98a32712008b46185cac18e16d7d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f31e0c37d242aaf430e25bd4a9f9e43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12a53d477ec1ce15ca7a3a52639d8a29 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-69 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2011-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b365a1be73650439359e11b9306811f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_224736426c3b91bf88751fdcbda92841
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_132c0d009fc016edede3d95bbf6b5713
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_083f3c9b5e9588ef7073153af701be4b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2043ebf2ed5d205b3d5f04c1cd575b32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7542d5513b0bce2e60982c4d911c3c7c |
| publicationDate |
2012-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012190073-A1 |
| titleOfInvention |
Process for large scale production of plasmid dna by e. coli fermentation |
| abstract |
The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of E. coli , including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmid DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10591463-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10626436-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014207166-A1 |
| priorityDate |
2004-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |