| abstract |
A viable global Na V 1.7 −/− knockout mouse is disclosed, and a breeding colony of global Na V 1.7 −/− knockout mice. Also disclosed are an isolated mouse gamete that does not encode a functional Na V 1.7 −/− , produced by the Na V 1.7 −/− knockout mouse; an isolated Na V 1.7 −/− mouse cell, or a progeny cell thereof, isolated from the Na V 1.7 −/− knockout mouse; and a primary cell culture or a secondary cell line and a tissue or organ explant or culture thereof derived from the Na V 1.7 −/− knockout mouse. Disclosed also are a hybridoma, wherein the hybridoma was originally formed from the fusion of the isolated Na V 1.7 −/− mouse cell mouse cell and a myeloma cell, and a method of making an antibody. Also disclosed are assays useful for screening prospective Na V 1.7 inhibitors and dose ranging a test Na V 17 inhibitor compound, which were validated using the Na V 1.7 −/− knockout mouse. |