| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5013b11264c17d055d44bf1b90682c71
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ede47b877b6df9c8d0c494cd66f45ae8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca60ed2a1975fa5f7a5fb641d82ebce8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a1d95e69b6a7159411c5f4a884160dc4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_507cfbd6d54778ff6c7390bb7215689a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1babf29192891ca1668fcb3936ab5eab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d14f3b2be1a4da6614defe90a541ecd |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2549-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2539-105 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2011-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e71703a64f0b43d95c9d0243842ef901
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a619a2206d9ede4eb11b31b3119daae
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86a7d2208f70ac3aad020bbd96c6a2be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13bc8308867427005fd32ca47f29393b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f4812217ee3273a58de4b94a62352ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d265017076eaaf99d18241cddb7f293 |
| publicationDate |
2012-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012135472-A1 |
| titleOfInvention |
Hot-start pcr based on the protein trans-splicing of nanoarchaeum equitans dna polymerase |
| abstract |
Disclosed is a hot-start PCR method, based on protein trans-splicing of intein-inserted large (Neq L) and small (Neq S) fragments of Neq DNA polymerase. The method comprises: preparing a PCR reaction mixture containing a sample DNA and primers; adding the Neq L fragment and the Neq S fragment together to the PCR reaction mixture, said Neq L fragment consisting of an amino acid sequence of SEQ ID NO: 2, with an intein amino acid sequence stretching from position 579 to 676 therein, said Neq S fragment consisting of an amino acid sequence of SEQ ID NO: 4 with an intein amino acid sequence stretching from position 1 to 30 therein; inducing the Neq L fragment and the Neq S fragment to undergo a protein trans-splicing process to form a polypeptide exhibiting Neq DNA polymerase activity; and performing a certain number of cycles of DNA denaturation, primer annealing and DNA extension. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106030629-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022263807-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9416352-B2 |
| priorityDate |
2010-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |