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filingDate 2012-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60c23544728daf815993a9ef2ff41108
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publicationDate 2012-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2012129157-A1
titleOfInvention Methods and Devices for Rapid Detection and Identification of Live Microorganisms by Aptamers and/or Antibodies Immobilized on Permeable Membranes
abstract The invention provides methods, devices and kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in about one hour or less.
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priorityDate 2007-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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