| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8472a512b6141ac2a1cd78cb0b1f7087
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b279ffb28d5cd5c2a0e62c66bc858f17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fbcce21e02d19bb1d1450e674042c947
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_734ae3e52ec22e67a9358a41c51ff1dc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cc726a73cb22b46b5ef1fcb42dd459d2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-325
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-545
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-7019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-163
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 |
| filingDate |
2011-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1aae152596506bea0bcf2cd01fbd437d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2816f1c6b58c207e885ec699e9e793a0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26049919639d189e271ec14c91c225be
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2eb98889c67d71b77101797be181ab7 |
| publicationDate |
2012-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012107848-A1 |
| titleOfInvention |
Polyclonal-monoclonal elisa assay for detecting n-terminus pro-bnp |
| abstract |
A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112710852-A |
| priorityDate |
2002-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |