http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012088290-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a036559e133cf9145349f432c9caee3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2efbe95965e682e179dfd266c9980505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b18b6ca959634fcbb61bf439940ef6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ae1706b02c14414fc41b80b134d06a95 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-582 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2010-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1223f7bca81d5706e928d7e8861e674c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69f79027fe69671dfe624451ecf8a5ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed10e8de298f40efdbb2f362d2565ad6 |
| publicationDate | 2012-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2012088290-A1 |
| titleOfInvention | Ethanol yield and reduction of biomass accumulation in the recombinant strain of saccharomyces cerevisiae overexpressing atpase |
| abstract | A new approach for increase of ethanol yield during alcoholic fermentation via decrease of biomass accumulation by using ATP degrading enzymes is described. The part of the Saccharomyces cerevisiae SSB1 gene coding for cytosolic ATPase domain of ribosome associated chaperon cloned into expression cassette under control of the glycerol-3-phosphate dehydrogenase gene (GPD1) promoter was introduced into the S. cerevisiae BY4742 strain. The recombinant strains were tested for their ability to grow and produce ethanol during glucose anaerobic and aerobic cultivations. Strains overexpressing ATPase domain of SSB1 possessed decreased concentration of intracellular ATP. They accumulated elevated amounts of ethanol and were characterized by decreased biomass accumulation as compared to the wild-type strain under both anaerobic and aerobic conditions. Similarly, the apyrase gene apy from E. coH encoding ATP/ADP hydrolyzing phosphatase and ATPase domain of SSB1 gene of S. cerevisiae were co-expressed under the control of galactose-inducible GAL1 promoter. The recombinant S. cerevisiae strains revealed slight reduction of biomass accumulation, while specific ATPase activity, ethanol accumulation and yield during alcoholic galactose fermentation under semi anaerobic conditions were increased. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7303908-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014100526-A1 |
| priorityDate | 2009-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 627.