Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cae467e0d8fcc350d461099fb93421aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41651508566f0385833e1ba14a21ffd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_97e76f7451a2e6493450ba006435944d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1670935e61565a1adc02491dcdbc22dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_69f74707ebb1302d9d8040d8ffbf8db5 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2011-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbc83e8ccf2e06dddd7e969235f650c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7127d5151ca3b8bfb29d538988c7bf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b43693bb91cd3c0ae64259dacb2fd841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9170fed12701571a968e19fba18a9642 |
publicationDate |
2012-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2012082979-A1 |
titleOfInvention |
Compositions, methods, and kits for (mis)ligating oligonucleotides |
abstract |
Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties. |
priorityDate |
2004-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |