| abstract |
A process for the enzymatic reduction of an enoate (1) wherein the C═C bond of the enoate (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate (2) in a system which is free of NAD(P)H, n n n n n n n n n n a. n b. in which n c. A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R═H or optionally substituted C 1 -C 6 -alkyl radical, n d. R 1 , R 2 and R 3 are independently of one another H, —O-C 1 -C 6 -alkyl , —O—W with W=a hydroxyl protecting group, C 1 -C 6 -alkyl, which can be substituted, C 2 -C 6 -alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or one of R 1 , R 2 and R 3 is a —OH radical, or R 1 is linked to R 3 so as to become part of a 4-8-membered cycle, or R 1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R 1 , R 2 and R 3 may not be identical. |