| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f62b36b442d05acbbbd8a50348777098
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da90def4dcb4567ccc802c14d8a67d4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26c7bbf63c9de2314ccfbd1639bc857b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate |
2011-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adf0e490e1220799f3a8fe32e51459d1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e48ae92c4ed0ccc606fff9e0ea7a7928 |
| publicationDate |
2012-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012034618-A1 |
| titleOfInvention |
Methods for culture and production of single cell populations of human embryonic stem cells |
| abstract |
We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019204750-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10138463-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8895300-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9598670-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019100940-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11015170-B2 |
| priorityDate |
2006-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |