| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6cc367bc90c1255f6e7df778eb4a694a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e268862df7e7104fbccb8f5a6b682eb |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B20-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate |
2011-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dedc5ab72eb60db7741c7466b82c38e3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f188a106f1906091b4c1c05eb45893b |
| publicationDate |
2012-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012028814-A1 |
| titleOfInvention |
Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing |
| abstract |
Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8741564-B2
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http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11414709-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11046721-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11021502-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11208688-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017051348-A1 |
| priorityDate |
2010-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |