| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d36619804ba2f4d6fe71f810dec75410
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07c9542186133097bd6bbb7d92393090
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb44c58b2fa788a96e49f5814a08a17e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5a3c61ce0d2ed842fa6a712ebf4a5a76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_51f13103ac56da911743cd9c24dadb09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ddd188925f99685ac6608aa4c6d7ed20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba495a5e81c727e02b2c45ccff47b028
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ddd511dd753e956a4c2261f45868b002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da7d9eef488cff28b2882e44dfcc22e0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4bbe230230ed47b22ae909eee7b9f647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5cd6d7c15c5ed6ddb6412308d9680ee |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-163333
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-7233
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-15 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B59-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B59-008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07B59-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C211-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C237-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 |
| filingDate |
2011-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_361ec84fd99667a9336ac7b484a526e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b08bd83fc6b684a923d535518d512854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f19c8af3f3c8b63dc7142ce24902ed43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8c01e432d94acd02b27211fc091130
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e49cdf85137ddef229adbef5b52f008c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44f74c5bd8e12a1c33af598191435707
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd00492abebdd84ff297c922d79d0e0b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e7666325abb6f15910d403e4105fb43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4e42ff362dd815a92459a04ab0e16f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d09ad00077ff8a879e65d133a332c0ea |
| publicationDate |
2012-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012022230-A1 |
| titleOfInvention |
Ionizable isotopic labeling reagents for relative quantification by mass spectrometry |
| abstract |
Relative quantification of metabolites by Electrospray Ionization Mass Spectrometry (ESI-MS) requiring a mechanism for simultaneous analysis of multiple analytes in two or more samples. Labeling reagents that are reactive to particular compound classes and differ only in their isotopic compositions facilitate relative quantification. Heavy and light isotopic forms of methylacetimidate were synthesized and used as labeling reagents for quantification of amine-containing molecules. Heavy and light isotopic forms of formaldehyde and cholamine were also synthesized and used independently as labeling reagents for quantification of amine-containing and carboxylic acid-containing molecules, such as found in biological samples. The labeled end-products are positively charged under normal acidic conditions involving conventional Liquid Chromatography Mass Spectrometry (LC/MS) applications. Labeled primary and secondary amine and carboxylic acid end-products generated higher signals concerning mass-spectra than pre-cursor molecules and improved sensitivity. Improved accuracy concerning relative quantification was demonstrated by mixing heavy and light labeled Arabidopsis extracts in different ratios. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9040903-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8455818-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9478405-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8742333-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11486367-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10852306-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9053916-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9698001-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011297823-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8530831-B1 |
| priorityDate |
2006-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |