http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012003638-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80f1de6dcceea098918a886d8bdf76d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f09c7c663de4f9fa65cad3b5970cc951 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5164a19624acf1df7770990b7d1413fb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef6644679d2981e221e13c0975052985 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6069f4272886a2c48586f4cfdfd37f92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6a0efb5163126bbde4791f1f40b712e |
| publicationDate | 2012-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2012003638-A1 |
| titleOfInvention | Kit for high throughput mutation screening methods |
| abstract | One example embodiment includes a kit to execute a method of simultaneously screening for genetic mutations in different genes in a multitude of samples. The kit includes an antisense deoxyribonucleic acid (DNA) probe, where the antisense DNA probe will be mixed with a strand of a ribonucleic acid (RNA) to be tested to form a heteroduplex molecule within a sample. The kit also includes a ribonuclease enzyme, an RNA-primed DNA polymerase, a single strand-specific nuclease, DNA-dependent DNA polymerase, a blocking adapter and a tagged reporter adapter. Through ribonuclease digestion, differential sequence fill-in (DSF) and full-length sequence extension, tagged mutant-dual adapter hybrids are formed for detection, quantification or amplification. The sequence ubiquity of said mutant-dual adapter hybrids enables the use of universalized primers for sequence amplification regardless of the numbers or the origins of the mutations involved. |
| priorityDate | 2007-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 121.