| abstract |
A test module for amplifying and detecting target nucleic acid sequences in a fluid, the test module having an outer casing with a receptacle for receiving a fluid containing the target nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. |