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filingDate 2011-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2011-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2011312648-A1
titleOfInvention Microfluidic device for genetic and mitochondrial analysis of a biological sample
abstract A microfluidic device for analysis of genetic DNA and mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria has a mitochondrial membrane containing mitochondrial DNA and the nuclei have a nuclear membrane containing nuclear DNA, a first lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, a second lysis section for lysing the nuclear membrane, mitochondrial probes downstream of the first lysis section for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, nuclear probes downstream of the second lysis second for hybridization with target nucleic acid sequences in the nuclear DNA to form probe-target hybrids.
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