| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53beb6661b97d439731d02a11e984a6d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50135123ba50354d7b10cf6830d48928
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f40660ed2da8b238cfadc62c5aa395f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e8f94fc0b0c4df69f9955db8b0701a4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2dc623a7b1bf611a297f9062148ca60f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-206
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-46 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01095
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate |
2011-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1ea2785a06ff642171693f7b9c87470
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f2b38b82f7654ff243e4da9ee2d6bdf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b54eec91b25dc4d544f20020f5b0ec2f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99e21a31455b645f04aceeafe331ec1c |
| publicationDate |
2011-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2011300580-A1 |
| titleOfInvention |
Selection of host cells expressing protein at high levels |
| abstract |
Described is a DNA molecule comprising an open reading frame sequence that encodes a selectable marker polypeptide, wherein the DNA molecule in the coding strand comprises a translation start sequence for the selectable marker polypeptide having a GTG start codon or a TTG start codon, and wherein the ORF sequence that encodes the selectable marker protein has been mutated to replace at least half of its CpG dinucleotides as compared to the native ORF sequence that encodes the selectable marker protein. Further provided are such DNA molecules wherein the ORF sequence that encodes a selectable marker polypeptide is part of a multicistronic transcription unit that further comprises an open reading frame sequence encoding a polypeptide of interest. Also described are methods for obtaining host cells expressing a polypeptide of interest, wherein the host cells comprise the DNA molecules described herein. Further provided is the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules described herein. |
| priorityDate |
2004-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |