| abstract |
The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR-type peptidyl prolyl cis-trans isomerase), Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component), RpoS (RNA polymerase sigma factor), PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins. |