| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_087bed7abab91ae0f927879f9fe8b273 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-14001 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate |
2011-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f377abe9eb2ca6cfa4f5b5daf668400
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f7fd163420706d3b47b8963dffe91bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb81c440fd4ccffaf577aa15b3da6b52 |
| publicationDate |
2011-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2011244537-A1 |
| titleOfInvention |
Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| abstract |
Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli , where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016007623-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9708587-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10100289-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10689627-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11279917-B2 |
| priorityDate |
2003-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |