http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011160457-A1

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filingDate 2009-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a8ca243bbef293c4577edfc3ba327a3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa66dcd5a9f32a8fa30950785ddeef4c
publicationDate 2011-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2011160457-A1
titleOfInvention Methods for obtaining cyclopamine
abstract The present invention relates to a process for extracting, purifying and isolating cyclopamine from cyclopamine-containing biomass comprising the steps of contacting cyclopamine-containing organic matter with an extractant (e.g., aqueous extractant) capable of extracting cyclopamine, separating the extracted organic matter from the cyclopamine/extract solution, concentrating the cyclopamine/extract solution leaving a concentrated aqueous solution, contacting the concentrated aqueous solution with an organic extractant capable of selectively extracting cyclopamine from the aqueous phase, concentrating the cyclopamine-containing organic phase, providing an enriched extract containing cyclopamine, separating cyclopamine from the enriched extract using column chromatography, providing at least one fraction containing cyclopamine, and crystallizing select chromatography fractions to provide purified cyclopamine.
priorityDate 2008-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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