Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b134c672fd4d3cd5b03362e45573990 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-475 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-475 |
filingDate |
2008-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd133a001f2e68791e64d489054400ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d09cf6ded71039f66da1d76fa4f87e18 |
publicationDate |
2011-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2011160441-A1 |
titleOfInvention |
Optimized purification process of recombinant growth factor protein |
abstract |
A process for the production of a purified recombinant GDF-5 related protein in prokaryotes comprises the steps of bacterial cell disruption and inclusion body solubilization to obtain a solubilized monomer of a GDF-5 related protein, said process characterized by a) disruption of bacterial cells with a high pressure homogenizer at a disruption pressure between 800 and 900 bar; and/or b) treatment of the recovered inclusion bodies with a denaturing solubilization buffer comprising L-arginine. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8945872-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9540429-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9914758-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9169308-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9359417-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9051389-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9631003-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8956829-B2 |
priorityDate |
2007-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |