Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate |
2009-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e46246f61b4c3ba46553df4efa97649 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2b8f1c472fafe991b14451c7ec818f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4e516ca8f9c21bf66d48bbf1d09a586 |
publicationDate |
2011-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2011159505-A1 |
titleOfInvention |
Method of Manufacturing a Mixture of Amplified Double-Stranded Nucleic Acids Comprising Unknown Sequence |
abstract |
The purpose of the subject invention is to provide a method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence including the complete 5′ end sequence, n A method of manufacturing a mixture of amplified double-stranded nucleic acids comprising: (a) preparing a single-stranded nucleic acid comprising a single-stranded adapter 1, a single-stranded nucleic acid fragment and a single-stranded adapter 2, and (b) conducting PCR with said single-stranded nucleic acid prepared in step (a), a primer 1, and a primer 2 to amplify double-stranded nucleic acids. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11760995-B2 |
priorityDate |
2008-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |