http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011151446-A1

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filingDate 2010-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a904bf393fc1b13e08af6ce1dee32def
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publicationDate 2011-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2011151446-A1
titleOfInvention Cell treatment solution and method of preparing stained cell suspension for a measurement of nuclear dna by flow cytometry
abstract A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the invention, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the invention includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye.
priorityDate 2009-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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