Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_87b20f1b762f11de373c4e6a9266814a |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-603 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-604 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-602 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 |
filingDate |
2008-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5cf3d8e1e96226803d09fa79607b6646 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e08df5263af0e1ead6fa42fefc4313fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_360f438c35745778c4f38dba003e203d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5673af8e6e1d7671d78c66514888528c |
publicationDate |
2011-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2011129928-A1 |
titleOfInvention |
Method of Manufacturing Induced Pluripotent Stem Cell Originated from Somatic Cell |
abstract |
Disclosed is a method for manufacturing stem cells including preparing Oct-4 gene, Sox2 gene, C-myc gene, and Klf-4 gene from mouse embryonic stem cells, and allowing each of the genes to be infected in host cells using a lentiviral vector system to generate viruses in which each of the genes are induced; concentrating or mixing each of the viruses to prepare a virus concentrated mixture, and mixing the virus concentrated mixture and a first culture solution to prepare a virus solution; floating mouse somatic cells having been cultivated in advance in a first culture dish, and mixing and reacting the floated somatic cells and the virus solution to prepare a somatic cell-virus mixture; adding and retaining the somatic cell-virus mixture as is in a second culture dish including a second culture solution to induce the genes in the somatic cells; and cultivating the somatic cells. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10195229-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013158890-A1 |
priorityDate |
2008-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |