http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011099648-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_161663de5408e572e0d7c6b04dfabc7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c576cf05543d136e60002c839947780 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-40 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2010-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61d42dd44cd70d1c0fdd2761f729d7f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2af4c37f3cb500d2723a5d72efe36d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb4f2ec73d261fe166436a56f9bf7229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44d1a8b1f8c9aa6d5b6ff9f8581a1938 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a76a639303490c38c9f692c6ee613b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb7cdcb404c4a95ace1838101ff64273 |
| publicationDate | 2011-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2011099648-A1 |
| titleOfInvention | Recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen stress and the use thereof |
| abstract | The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp β-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp β-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream ( Megalobrama amblycephala ) and common carp ( Cyprinus carpio L.), it enhances their hypoxia tolerance as well. Such genetically improved breeding technique may be widely used for breeding new excellent farmed species with the hypoxia tolerance. |
| priorityDate | 2009-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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