| abstract |
The invention relates to a method for separating fractions of a mixture to be separated comprising the steps ofn providing a device comprising at least two chromatography columns mounted in series, discontinuously injecting a mixture to be separated, in a single point of the device, discontinuously drawing off the fraction enriched in the product of interest, in a single point of the device, injecting eluents in each column and displacing the points for injecting eluents, the eluents having a different eluting power. nn The method allows efficient separation which is simple to perform. |