| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a3a5fcca917003260c614f5baacc148
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2799e5e0025de9c329a18b5317423c1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fe9f1c3f3490b0fd9eb2d3058fbeb5cf |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-527 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-527 |
| filingDate |
2010-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ac0057292e8045a74387e0e50eae4c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af6d4b586a50da1827a4d69ecb26d760
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de89e54417f546fe97893181382220b4 |
| publicationDate |
2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2011021767-A1 |
| titleOfInvention |
B12-Dependent Dehydratases With Improved Reaction Kinetics |
| abstract |
Sequences of B 12 -dependent dehydratases with improved reaction kinetics are presented. Use of these B 12 -dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays. |
| priorityDate |
2002-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |