Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c3e16d64f084e069649f49c5a7ab584 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_91d37cede3e2888e42987b8ae96cbb3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8892c7511c955c596c3da482671b4c2c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7090a787ddf1ae4f13874da265166f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_095381c02c80bfac351aaae98315af84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1b2460cb29f51d9cc72e83f4396a37c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd97e10ff64ab601fd3187de8d9b152c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2274f1d630f9ba310ce52df59241717f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f4b4c006c77765597f2d4a894ce86fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_149b94c5093f3489fa847f06e4696dc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c438f0fa9a9f173dacaee14ee347091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1922236adc2029d4b61ec288b547ee0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7ab254ce97aa5d2683f7d196ca232f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06c98e1178136ac6011937e24ef16fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c147327252a9e5b2348b1540ba4f8dbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfdfe5771f9105ea7a50e75739e0d268 |
publicationDate |
2010-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2010291570-A1 |
titleOfInvention |
Materials and methods for detection of nucleic acids |
abstract |
Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011097764-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8440406-B2 |
priorityDate |
2000-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |