| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb1a345d690bc8bd1548ab004b6589ce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9257cfbf3498192fb12713c7c306233
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da0723f795336232c476541d0341bf78 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-28 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0691
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5064
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5091 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-00 |
| filingDate |
2006-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e6710935a794bded702b392de879e8b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f09408265be3ac2a84997d5dbf786476
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea48685024055c76b99d7e7729745afe |
| publicationDate |
2010-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2010273200-A1 |
| titleOfInvention |
In-Vitro Model of Blood-Brain Barrier, In-Vitro Model of Diseased Blood-Brain Barrier, and Drug Screening Method, Analysis Method for Functions of Diseased Blood-Brain Barrier, and Analysis Method for Pathogenesis Using the Same |
| abstract |
It is intended to provide a screening system for a centrally acting drug transported across the blood-brain barrier, a drug acting on the blood-brain barrier itself, or a drug transferred into the brain without being expected to centrally act. Moreover, another object of the present invention is to achieve pathogenesis analysis study or the screening in a diseased state by applying various diseased environments to this screening system. The present invention provides an in-vitro model of blood-brain barrier obtained by using a three-dimensional culture apparatus comprising: a culture solution; a plate holding the culture solution; and a filter immersed in the culture solution and placed in no contact with the inside bottom of the plate, the filter having plural pores of 0.35 to 0.45 μm in diameter, and by comprising: seeding primary cultured brain capillary endothelial cells onto the upper surface of the filter; seeding primary cultured brain pericytes onto the under surface of the filter; seeding primary cultured astrocytes onto the inside surface of the plate; and coculturing these cells in a normal culture solution. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11447752-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019178317-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11612150-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10947500-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9932559-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11732023-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10143187-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014142370-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10457717-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104164365-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11786559-B2 |
| priorityDate |
2005-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |