http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010221763-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6636b59c3149a23a6ae7feaa3984d05f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91188 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91091 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91194 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H3-06 |
| filingDate | 2006-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d57beff7a1315f26bd2cac22c96e1fa6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60435c728d752e8d3f43b76601bd8198 |
| publicationDate | 2010-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2010221763-A1 |
| titleOfInvention | Specific Acceptors for Transferases to Saccharides and Method for Obtaining and Using Same |
| abstract | A method for determining substrates specific for a transferase enzyme selected from the group consisting of glycosyltransferases and sulfotransferases. The method includes the steps of:n a) selecting a substrate to be tested for specificity for sulfotransferases and for glycosyltransferases, from tri, tetra or penta saccharide compounds containing at least one →GlcNAcβ1 saccharide that may be substituted with MeO—, EtO—, Allyl-O, or N 3 — and terminating in →3GalNAcα-OR, →3GlcNAcβ-OR or →3Galα-O—R where R is alkyl, aryl, azido or allyl all of 1 through 8 carbon atoms where R may be substituted with halo, —OH, lower alkyl, —SO 3 , or —NO 2 ; b) combining the substrate separately with each glycosyltransferase or sulfo-transferase in a series, for transfer of a particular glycosyl or sulfo moiety, to a saccharide, in conjunction with a donor compound for the particular moiety, where the moiety is radio labeled, in a buffered aqueous solution of about pH of about 6 to about 7, to form incubation mixtures; c) incubating the incubation mixtures at a temperature of from about 18 to about 40 degrees Celcius for from about 30 minutes to about four hours; d) testing the resulting incubation mixtures of each separate glycosyltransferase or sulfotransferase for reaction product comprising a chemical combination of substrate and particular moiety to determine effectiveness of each separate glycosyltransferase or sulfotransferase in transferring the particular moiety to the substrate; and e) comparing the effectiveness of each glycosyltransferase or sulfotransferase in transferring the particular moiety to determine whether a particular glycosyltranferase or sulfotransferase acts to transfer the particular moiety to the substrate when the other glycosyltransferases or sulfotransferases do not do so, thus determining whether the substrate acts specifically with a particular glycosyltransferase or sulfotransferase. nn The invention also includes a method for the specific detection of a specific transferase for a specific moiety selected from sulfotransferases and glycosyltransferases. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10633454-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10633665-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019213069-A1 |
| priorityDate | 2005-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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