http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010221719-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b06e6bf3a51c442570e5046863a5cd8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e80cba577e5a025c3d333578f9905e9 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5035 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2010-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3639dbb29d34bd1ae715d48ed558a74e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23245fb06a5e74edb9f301a92b0daf1a |
publicationDate | 2010-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2010221719-A1 |
titleOfInvention | Probes for detecting protein nuclear transport and method for detecting and quantifying protein nuclear transport using the same |
abstract | A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order. |
priorityDate | 2004-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 218.