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publicationDate 2010-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2010204059-A1
titleOfInvention CDR-Anchored Amplification Method
abstract A method of obtaining nucleic acid encoding a plurality of antibodies is provided. In certain embodiments, the method comprises obtaining from an immunized animal nucleic acid encoding the amino acid sequence of the heavy and light chains of a second antibody that binds to the antigen as a first antibody and differs in amino acid sequence to the first antibody, wherein the obtaining is done by amplification using: i. a first primer pair that includes oligonucleotides are complementary to CDR-encoding regions first antibody.
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