http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010190209-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30ef8e25c57d9c930fb7e4aec5e674e5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-51 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2009-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdda870e3cbd2449a604e1610950e2db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6eb8c35c00a920315db8b3919922d976 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2ba44d872888501416df8b091472d9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e94141e31aaab019026947e5e731aff |
| publicationDate | 2010-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2010190209-A1 |
| titleOfInvention | Optimized dna sequences encoding recombinant human bone morphogenetic protein-2 (rhbmp-2), preparation method and the uses thereof |
| abstract | The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification. |
| priorityDate | 2009-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 233.