| abstract |
Method for determining the content of a non-fluorescent chromophorous first compound, in a biological tissue including a fluorescent chromophorous second compound, the method includes at least one iteration of the following operations:n emission, in the direction of said tissue, of a first reference optical radiation, and a second measurement optical radiation, each chosen so as to induce a fluorescence radiation of the second compound, each of the first and second radiations being partially absorbed by the first compound, measuring the fluorescence radiations induced by each of the first and second radiations, and determining, from the measurement, of the content of the first compound in the tissue, wherein the method includes at least one compensation of a measurement saturation due to too high an absorption of the measurement radiation by the first compound, the compensation comprising a choice, for the measurement radiation, of a wavelength corresponding to a lower absorption in the absorption spectrum of the first compound. |