http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010167326-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5547f741b25666fc4ae5195cf71a979b |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6821 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-128 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-107 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
filingDate | 2007-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e12f9884ccf0945a30dd916ac0dd7689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_494383d9b1da5c6bdd5de9d421612f9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61e9b336eed5d596ce475688401b81bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0220d020284f4abb988ceb8d40be158 |
publicationDate | 2010-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2010167326-A1 |
titleOfInvention | Method for cleavage of peptidic bond at c terminal of peptide and a method for determination of c terminal amino acid sequence of peptide |
abstract | The present invention is to provide a method for cleavage of peptidic bond at C terminal of peptide and a method for determination of C terminal amino acid sequence of peptide without the decrease of the sensitivity and with preventing the subsidiary reaction. The method for cleavage of peptidic bond at C terminal of peptide according to the present invention is characterized as follows:n A method for cleavage of peptidic bond at C terminal of peptide comprising the steps of: reacting a peptide with a solution of perhalogenated carboxylic acid containing alcohol to esterify glutamic acid residue of said peptide; and reacting said peptide with alkanoic acid anhydride to obtain C terminal-deleted peptide in which the amino acid residue of said peptide at C terminal is sequentially deleted. The method for determination of C terminal amino acid sequence of peptide according to the present invention is characterized as follows: A method for determination of C terminal amino acid sequence of peptide comprising the steps of: reacting a peptide with a solution of perhalogenated carboxylic acid containing alcohol to esterify glutamic acid residue of said peptide; reacting said peptide with alkanoic acid anhydride to obtain C terminal-deleted peptide in which the amino acid residue of said peptide at C terminal is sequentially deleted; measuring molecular weight of said C terminal-deleted peptide; and grasping said peptide based on said molecular weight. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10877043-B2 |
priorityDate | 2006-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 192.