| abstract |
Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes . These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria. |